Structured around seven compelling themes, this Research Handbook examines the foundations, the mindset, voice and practice of key actors, and distinct cross-cultural contexts of entrepreneurial ecosystems. Chapters consider the important role of academic entrepreneurship before exploring the governance and evolution of digital entrepreneurship and sustainable entrepreneurial ecosystems. They conclude by addressing one of the most challenging practice and policy issues, the measurement of entrepreneurial ecosystems. Adopting a broad contextualization approach that draws on empirical insights and perspectives from an array of geographical, institutional and cultural contexts, it paves the way for promising future research avenues in entrepreneurial ecosystems.